Biotin-14-dATP is a dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP. Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase. The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation.
Applications:
Incorporation into DNA/cDNA by
– Nick Translation with DNAse I/ DNA Polymerase I
– Primer Extension with Klenow fragment
Specifications:
Concentration: 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. |
Protocol (PDF): | C431 | |
MSDS (PDF): | MSDS-C431 |
References:
Novel biotinylated nucleotide-analogs for labeling and colorimetric detection of DNA.
Gebeyehu et al.
Nucleic Acids Res. (1987) 15 (21):4513
Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell.
Nagano et al.
Nature Protocols (2015) 10 (12):1987.
HiChIP: Efficient and sensitive analysis of protein-directed genome architecture.
Mumbach et al.
Nature Protocols (2016) 13 (11):919.
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