PolyFectRNA™ Transfection Reagent

PolyFectRNA™ Transfection Reagent is an innovative cationic nanotechnology developed specifically for the delivery of siRNA and miRNA into a wide range of eukaryotic cells. PolyFectRNA™ Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. The reagent is compatible with the use of serum and antibiotics in the cell culture medium.  

Features

• A simple and rapid protocol for consistent and reproducible results
• Superior transfection efficiency
• Compatible with a broad range of cell types
• Low cytotoxicity

Figure 1.  HeLa cells stably expressing the GFP were transfected with either a negative control siRNA (Left), or an anti-GFP siRNA (right) with PolyFectRNA™ Transfection Reagent. The gene silencing efficiencies were assessed by GFP signal. The GFP signal was not inhibited (<2%) when transfected with a negative control siRNA. When transfected with an anti-GFP siRNA, the GFP signal was significantly inhibited (>90%).

Figure 2.  A549 cells stably expressing the GL3 luciferase were transfected with either a negative control siRNA, or a GL3Luc siRNA at 10 or 25 nM with PolyFectRNA™ Transfection Reagent. The luciferase activity, determined 48 hours post-transfection, was inhibited up to 92% when the transfection was performed with 10 to 25 nM siRNA. As a negative control, the luciferase activity was not inhibited in the same conditiond with NC siRNA.