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EasySC DNA Purification Kit from bacteria

Introduction

EasySC DNA Purification Kit from bacteria provides a rapid and easy method for the isolation of high-quality genomic DNA from a wide variety of gram-positive and negative bacterial species. This kit uses an optimized lysis condition and up to 1 x 109 bacterial cells can be processed for each column. The buffer system is optimized to allow direct cell lysis followed by selective binding of DNA to the DNA column membrane. Simple centrifugation processing completely removes contaminations and enzyme inhibitors such as proteins and divalent cations, and allows simultaneous processing of multiple samples in parallel.  Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion.


Features

  • Fast – DNA purification process in less than 20 min post-lysis.
  • Safe – No organic extraction, no ethanol precipitation.
  • High-quality – DNA is suitable for a variety of downstream applications.

 

Technical Specifications

Applications Genomic DNA isolation from gram-positive and negative bacterial cells.
Sample Source Bacterial cells
Sample Input 1 x 10cells
Elution Volume 50-100 μL
Technology Silica spin column
Processing Mode Manual, Centrifugation/Vacuum

 

Figure 1.  Genomic DNA from E. coli cells was isolated using ABP EasySC DNA Purification Kit from bacteria. Eluted DNA was analyzed on a 1% agarose gel. 

To Order

Buy Cat.No. Product Name Unit Size Price
D119-1 EasySC DNA Purification Kit from bacteria 50 preps $165
D119-2 EasySC DNA Purification Kit from bacteria 200 preps $495

Documents

Protocol (PDF):

D119

MSDS (PDF): 

Buffer ATL                Buffer DL

Buffer AW1              Buffer AW2

Buffer AE                 Proteinase K