Introduction
EasyMag Plasmid Mini Purification Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with magnetic beads technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
Features
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Rapid – Purification of plasmid DNA in less than 30 minutes.
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Safe – No Phenol/chloroform extractions.
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High-quality – DNA is suitable for a variety of downstream applications.
Technical Specifications
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Applications
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Plasimd DNA isolation from 1-6 mL bacterial cultures.
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Sample Source
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LB culture
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Sample Input
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1-6 mL high-copy plasimd, 10 mL low-copy plasimd
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Elution Volume
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30-100 μL
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Technology
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Magnetic Bead
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Processing Mode
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Manual, Automated
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Figure 1. 5 µL of purified pUC18 plasmid was analyzed on a 1% Agarose gel. Plasmid was isolated from 5 mL DH5α cultures harboring the plasmid using ABP EasyMag Plasmid Mini Purification Kit.
Table 1. pUC18 plasmid DNA yield and quality using ABP EasyMag Plasmid Mini Purification Kit