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EasyMag Plasmid Mini Purification Kit

Introduction

EasyMag Plasmid Mini Purification Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with magnetic beads technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.


Features

  • Rapid – Purification of plasmid DNA in less than 30 minutes.
  • Safe – No Phenol/chloroform extractions.
  • High-quality – DNA is suitable for a variety of downstream applications.

 

Technical Specifications

Applications Plasimd DNA isolation from 1-6 mL bacterial cultures.
Sample Source LB culture
Sample Input 1-6 mL high-copy plasimd, 10 mL low-copy plasimd
Elution Volume 30-100 μL
Technology Magnetic Bead
Processing Mode Manual, Automated

 

Figure 1.   5 µL of purified pUC18 plasmid was analyzed on a 1% Agarose gel. Plasmid was isolated from 5 mL DH5α cultures harboring the plasmid using ABP EasyMag Plasmid Mini Purification Kit.
 

 

Table 1. pUC18 plasmid DNA yield and quality using ABP EasyMag Plasmid Mini Purification Kit

To Order

Buy Cat.No. Product Name Unit Size Price
D101-1 EasyMag Plasmid Mini Purification Kit 50 preps $55
D101-2 EasyMag Plasmid Mini Purification Kit 200 preps $180

Documents

Protocol (PDF):

D101

MSDS (PDF): 

Buffer P1                     Buffer P2

Buffer N3                    Buffer PW

Elution buffer            RNase A