EasySC Plasmid Mini Purification Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with Spin Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
- Rapid – Purification of plasmid DNA in less than 30 minutes.
- Safe – No Phenol/chloroform extractions.
- High-quality – DNA is suitable for a variety of downstream applications.
|Applications||Plasimd DNA isolation from 1-6 mL bacterial cultures.|
|Sample Source||LB culture|
|Sample Input||1-6 mL high-copy plasimd, 10 mL low-copy plasimd|
|Elution Volume||30-100 μL|
|Technology||Silica spin column|
|Processing Mode||Manual, Centrifugation/Vacuum|
Figure 1. 5 µL of purified pUC18 and pUC19 plasmids were analyzed on a 1% Agarose gel. Plasmid was isolated from 5 mL DH5α cultures harboring the plasmid using ABP EasySC Plasmid Mini Purification Kit.
Table 1. DNA yield and quality using ABP EasySC Plasmid Mini Purification Kit
- Genomic DNA Purification
- RNA Purification
- Viral DNA & RNA Purification
- Plasmid DNA Purification
- PCR & Gel Cleanup
- Spin Column & Magnetic Beads
- DNA & RNA Gel Electrophoresis
- DNA & RNA Quantitation