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EasySC Plasmid Mini Purification Kit

Introduction

EasySC Plasmid Mini Purification Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with Spin Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.


Features

  • Rapid – Purification of plasmid DNA in less than 30 minutes.
  • Safe – No Phenol/chloroform extractions.
  • High-quality – DNA is suitable for a variety of downstream applications.

 

Technical Specifications

Applications Plasimd DNA isolation from 1-6 mL bacterial cultures.
Sample Source LB culture
Sample Input 1-6 mL high-copy plasimd, 10 mL low-copy plasimd
Elution Volume 30-100 μL
Technology Silica spin column
Processing Mode Manual, Centrifugation/Vacuum


Figure 1.   5 µL of purified pUC18 and pUC19 plasmids were analyzed on a 1% Agarose gel. Plasmid was isolated from 5 mL DH5α cultures harboring the plasmid using ABP EasySC Plasmid Mini Purification Kit.
 

Table 1. DNA yield and quality using ABP EasySC Plasmid Mini Purification Kit

 

To Order

Buy Cat.No. Product Name Unit Size Price
D100-1 EasySC Plasmid Mini Purification Kit 50 preps $55
D100-2 EasySC Plasmid Mini Purification Kit 200 preps $180

Documents

Protocol (PDF):

D100

MSDS (PDF): 

Buffer P1                     Buffer P2

Buffer N3                    Buffer PW

Elution buffer            RNase A