Biotin DBCO

Introduction

Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. DBCO alkynes can be used to perform click reactions with azide-modified targets without the use of heavy metal catalysis. DBCO reactions are ideal for surface labeling of live cells and also minimize damage to fluorescent proteins like GFP or R-PE.
The biotin DBCO is reactive with azide via a Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC). Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.

Features

Efficiency—the click reaction is complete in less than 1 hour;
Specificity—the reaction between the label and detection tag is selective and specific;
Stability—the reaction product contains an irreversible, covalent bond;
Biologically inert—the components of the reaction do not undergo any side reactions.

Figure 1. Click chemistry labeling

Specifications

Label:	Biotin
Ex/Em:	–
Detection Method:	–
Solubility:	DMSO, DMF
Molecular Weight:	747.95
Product Size:	1 mg
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications Click chemistry labeling

To order

Documents

Datasheet (PDF):	C330
MSDS (PDF):	C330

Reference

Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.

Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,
Angew Chem Int Ed Engl (2012) 51:3143-3146

Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry.

Winz ML, Samanta A, Benzinger D, Jäschke A,
Nucleic Acids Res (2012) 40:e78-e78

Related products

Buy	Cat.No.	Product Name	Ex/Em(nm)	Unit	Price
	C330	Biotin DBCO	–	1 mg	$200

Features

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