BCA Protein Assay Kit | ABP Biosciences

Introduction

The BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure total protein concentration compared to a protein standard. The BCA Protein Assay Kit is based on the formation of a Cu²⁺-protein complex under alkaline conditions, followed by reduction of the Cu²⁺ to Cu¹⁺ by cysteine, cystine, tryptophan, tyrosine, and peptide bonds. BCA forms a purple colored complex with Cu¹⁺ that is detectable at 562nm, and proportional to the protein present. The BCA Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Features

Colorimetric: measure with a standard spectrophotometer or plate reader at 562nm
Excellent uniformity: less protein-to-protein variation
Compatible: unaffected by typical concentrations of most ionic and nonionic detergents
Moderately fast: much easier and faster than the Lowry method
High linearity: linear working range for BSA equals 20 to 2000 µg/mL
Sensitive: detect down to 5 µg/mL with the enhanced protocol

Applications

Protein quantitation in solution.

To Order

Documents

Protocol (PDF):	P011
MSDS (PDF):	MSDS-P011
COA (PDF):	COA-P011

Cited Reference:

Bergamottin reduces liver damage by suppressing inflammation, endoplasmic reticulum and oxidative stress in cafeteria diet-fed mice
E Yazici, E Sahin, E Alvuroglu, E Yulug, A Mentese
Food Bioscience, 2023, 52, 102371. https://doi.org/10.1016/j.fbio.2023.102371

Acetylbritannilactone attenuates contrast-induced acute kidney injury through its anti-pyroptosis effects.
Fei Chen, Jingchao Lu, Xiuchun Yang, Bing Xiao, Huiqiang Chen, Weina Pei, Yaqiong Jin, Mengxiao Wang, Yue Li, Jie Zhang, Fan Liu, Guoqiang Gu, Wei Cui
Biosci Rep 2020; 40 (2): BSR20193253. doi: https://doi.org/10.1042/BSR20193253

Fusobacterium nucleatum Causes Microbial Dysbiosis and Exacerbates Visceral Hypersensitivity in a Colonization-Independent Manner
Gu X, Song L, Li L, Liu T, Zhang M, Li Z, Wang P, Li M and Zuo X
Front. Microbiol. 2020, 11:1281. doi: 10.3389/fmicb.2020.01281

TRIM31 enhances chemoresistance in glioblastoma through activation of the PI3K/Akt signaling pathway
Fan MD, Zhao XY, Qi JN, Jiang Y, Liu BY, Dun ZP, Zhang R, Wang CW and Pang Q
Exp Ther Med 20: 802-809, 2020. https://doi.org/10.3892/etm.2020.8782

Probiotic Interventions Alleviate Food Allergy Symptoms Correlated with Cesarean Section: A Murine Model
Jin B-Y, Li Z, Xia Y-N, Li L-X, Zhao Z-X, Li X-Y, Li Y, Li B, Zhou R-C, Fu S-C, Li S-Y and Li Y-Q
Front. Immunol. 2021, 12:741371. doi: 10.3389/fimmu.2021.741371

Tilapia skin peptides, a by-product of fish processing, ameliorate DSS-induced colitis by regulating inflammation and inhibiting apoptosis
Gao J, Li L, Zhao D, Wang X, Xia Y, Li B, Liu C and Zuo X
Front. Nutr. 2022, 9:988758. doi: 10.3389/fnut.2022.988758

Hsa_circ_0001021 regulates intestinal epithelial barrier function via sponging miR-224-5p in ulcerative colitis
Bing Li, Yan Li, Lixiang Li, Yu Yu, Xiang Gu, Chang Liu, Xin Long, Yanbo Yu & Xiuli Zuo
EPIGENOMICS, 2021, 13, 17. https://doi.org/10.2217/epi-2021-0230

Chemical characterization, gastrointestinal motility and sensory evaluation of dark chocolate: A nutraceutical boosting consumers' health
Caponio, G.R.; Lorusso, M.P.; Sorrenti, G.T.; Marcotrigiano, V.; Difonzo, G.; De Angelis, E.; Guagnano, R.; Ciaula, A.D.; Diella, G.; Logrieco, A.F.; Montagna, M.T.; Monaci, L.; De Angelis, M.; Portincasa, P.
Nutrients 2020, 12(4), 939; https://doi.org/10.3390/nu12040939

Antimicrobial and Anticancer Activity of Corydalis solida
Dulger G.
Saglık Bilimlerinde Deger. 2022; 12(3): 534-539. https://doi.org/10.33631/sabd.1145694

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	P011	BCA Protein Assay Kit	Protein quantitation	500 mL	$105