Introduction
	Our Apoptotic, Necrotic, and Healthy Cells Detection Kit provides a convenient assay to detect apoptotic, necrotic, and total cells within the same cell population by flow cytometry or fluorescence microscopy. This kit contains three-color reagent:  Andy Fluor™ 488 annexin V used to stain apoptotic cells by binding to phosphatidylserine (PS) exposed on the cell surface; propidium iodide (PI) used to stain necrotic cells with comprised membrane integrity; and Hoechst 33324 used to stain all cell nuclei. After staining a cell population, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and all cells show blue fluorescence.
	 
	Features:
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			Simple: Simultaneous detection of apoptotic, necrotic, and total cells in a single assay
		
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			Fast: A single 30 min staining step
		
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			Sensitive: Brighter fluorescence signal
		
 
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									Specifications
 
| Platform: | Fluorescence Cytometer, Fluorescence Microscopy |  
| Detection Method: | Fluorescent |  
| Ex/Em: | PI: 535/617 nm; Andy Fluor™ 488: 495/520 nm; Hoechst 33324: 350/460 nm |  
| Product Size: | 50 assays |  
| Storage Conditions: | 4 ℃, protect from light |  
| Shipping Condition: | Ice pack |  |  |  | 
	
 
Documents
	 
	
	Cited Reference:
	Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
	Sema Serter Kocoglu, Mücahit Seçme, Levent Elmas
	Arch Biol Sci. 2022;74(3):227-234; https://doi.org/10.2298/ABS220219021S
	Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
	Serter Koçolu S, Seçme M, Elmas L.
	Arch Biol Sci 2022, 74(3):227-34. https://doi.org/10.2298/ABS220219021S
	Copper Nanoparticles Induce Oxidative Stress via the Heme Oxygenase 1 Signaling Pathway in vitro Studies
	Zou L, Cheng G, Xu C, Liu H, Wang Y, Li N, Fan X, Zhu C, Xia W
	Int J Nanomedicine. 2021, 16:1565-1573. doi: 10.2147/IJN.S292319
	Activation of Aquaporin 5 by carcinogenic Helicobacter pylori infection promotes epithelial-mesenchymal transition via the MEK/ERK pathway
	Li, N., Xu, X., Yang, H., Wang, H., Ouyang, Y., Zhou, Y., … Hong, J.
	Helicobacter. 2021,00:e12842. https://doi.org/10.1111/hel.12842
	Fresh and cultured mouse islets differ in their response to nutrient stimulation
	Morsi M, Schulze T, Früh E, Brüning D, Panten U, Rustenbeck I.
	Endocr Connect. 2020, 9(8):769-782. doi: 10.1530/EC-20-0289
	Naringin  induces  endoplasmic  reticulum  stress-mediated  apoptosis,  inhibits  catenin  pathway  and  arrests  cell  cycle  in  cervical  cancer  cells
	Ruyin Lin, Xinxin Hu, Shaorong Chen, Qiyang Shi and Huiqing Chen
	Acta Biochimica Polonica, 2020, 67, 181-188. https://doi.org/10.18388/abp.2020_5182
	Pharmacological inhibition of thioredoxin reductase increases insulin secretion and diminishes beta cell viability
	Brüning, D., Hatlapatka, K., Lier-Glaubitz, V. et al.
	Naunyn-Schmiedeberg's Arch Pharmacol 394, 1133–1142 (2021). https://doi.org/10.1007/s00210-020-02046-2
	MicroRNA function can be reversed by altering target gene expression levels
	Alexander A. Svoronos, Stuart G. Campbell, Donald M. Engelman
	iScience, 2021, 24, 10, 103208, https://doi.org/10.1016/j.isci.2021.103208
	Monensinin Glioblastoma Multiformede Kaspaz-10 Aracılı Apoptoz Üzerine Etkileri
	Sema SERTER KOÇOLU, Mücahit SEÇME
	Uluda Üniversitesi Tıp Fakültesi Dergisi, 47 (3) 393-397, 2021. DOI: https://doi.org/10.32708/uutfd.999511
	[11]-Chaetoglobosins with Cytotoxic Activities from Pseudeurotium Bakeri
	Ruan, Hanli and Duan, Fangfang and Gao, Ying and Peng, Xiaogang and Meng, Xianggao and Chang, Jinling and Gan, Yutian and Ouyang, Qianxi
	SSRN: http://dx.doi.org/10.2139/ssrn.4102564