Recombinant Human Angiotensin-Converting Enzyme 2/ACE-2 protein produced by transfected human cells is a secreted protein with sequence (Ser19-Asp615) of human ACE-2.
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Protein Construction:
A DNA sequence encoding the human ACE2 Protein (Ser19-Asp615) was expressed.
Species:
Human.
Expressed Host:
HEK293 Cells.
Purity:
> 95% as determined by SDS-PAGE.
Endotoxin:
< 1.0 EU per μg protein as determined by the LAL method.
Predicted N Terminal:
Ser.
Molecule Weight:
The recombinant human ACE2 Protein consists of 597 amino acids and predicts a molecular mass of 69.1 kDa.
Protein Storage Buffer:
150 mM NaCl, 20 mM NaHCO3, pH 7.0.
SDS-PAGE:
Reference:
1. Koitka A, et al. (2008) Angiotensin converting enzyme 2 in the kidney. Clin Exp Pharmacol Physiol. 35(4): 420-5.
2. Raizada MK, et al. (2007) ACE2: a new target for cardiovascular disease therapeutics. J Cardiovasc Pharmacol. 50(2): 112-9.
3. Imai Y, et al. (2007) Angiotensin-converting enzyme 2 (ACE2) in disease pathogenesis. Circ J. 74(3): 405-10.
