Recombinant Human ACE2 Protein

Recombinant Human Angiotensin-Converting Enzyme 2/ACE-2 protein produced by transfected human cells is a secreted protein with sequence (Ser19-Asp615) of human ACE-2.

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

Protein Construction:

A DNA sequence encoding the human ACE2 Protein (Ser19-Asp615) was expressed.

Species:

Human.

Expressed Host:

HEK293 Cells.

Purity:

> 95% as determined by SDS-PAGE.

Endotoxin:

< 1.0 EU per μg protein as determined by the LAL method.

Predicted N Terminal:

Ser.

Molecule Weight:

The recombinant human ACE2 Protein consists of 597 amino acids and predicts a molecular mass of 69.1 kDa.

Protein Storage Buffer:

150 mM NaCl, 20 mM NaHCO₃, pH 7.0.

SDS-PAGE:

Reference:

1. Koitka A, et al. (2008) Angiotensin converting enzyme 2 in the kidney. Clin Exp Pharmacol Physiol. 35(4): 420-5.

2. Raizada MK, et al. (2007) ACE2: a new target for cardiovascular disease therapeutics. J Cardiovasc Pharmacol. 50(2): 112-9.

3. Imai Y, et al. (2007) Angiotensin-converting enzyme 2 (ACE2) in disease pathogenesis. Circ J. 74(3): 405-10.