Introduction To order Documents Related products Introduction Internucleosomal cleavage of DNA is a hallmark of apoptosis. DNA cleavage in apoptotic cells can be detected in situ in fixed cells or tissue sections using the terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) method. TUNEL is highly selective for the detection of apoptotic cells but […]
Continue Reading
Home » Articles posted byYLQ (Page 47)
Author Archives: YLQ
TUNEL assays
Introduction To Order Related products Introduction The later stages of apoptosis are characterized by changes in nuclear morphology, including DNA fragmentation, chromatin condensation, degradation of nuclear envelope, nuclear blebbing, and DNA strand breaks. DNA fragmentation that occurs during apoptosis produces DNA strand breaks, which can be analyzed using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end-labeling) […]
Continue Reading
Mitochondrial Membrane Potential Assays
Introduction To order Related products Introduction A distinctive feature of the early stages of apoptosis is the disruption of active mitochondria, including changes in membrane potential and alterations to redox potential. JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide) is a novel cationic carbocyanine dye. In healthy cells, JC-1 selectively accumulates in mitochondria and forms J-aggregates that exhibit red fluorescence. […]
Continue Reading
JC-1 Mitochondrial Membrane Potential Detection Kit
Introduction To order Documents Related products Introduction JC-1 Mitochondrial Membrane Potential Detection Kit provides a quick and reliable method to detect the mitochondrial membrane potential changes in apoptotic cells using flow cytometry. The loss of mitochondrial membrane potential is a hallmark for apoptosis. The JC-1 Assay Kit uses a unique cationic dye (5,5’,6,6’- tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide) to signal […]
Continue Reading
Caspase-3/7 Inhibitor Ac-DEVD-CHO
Introduction To order Documents Related products Introduction Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for caspase-3/7. It contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibitor caspase-3/7 activity in apoptotic cells, and to study events downstream of caspase-3/7 activation. Substrate Specifications Substrate name: Ac-DEVD-CHO Molecular weight: 502.5 […]
Continue Reading
Caspase-3/7 Substrate Z-DEVD-R110
Introduction To order Documents Related products Introduction The caspase-3/7 substrate is a bisamide derivative of the fluorophore rhodamine 110 (R110). Peptides are covalently linked to each of the amino groups of rhodamine 110 (R110), thereby suppressing both the visible absorption and fluorescence of the dye. Upon enzymatic cleavage, the nonfluorescent bisamide substrate is converted in […]
Continue Reading Caspase-3/7 Z-DEVD-R110 Assay Kit
Introduction To order Documents Related products Introduction The Caspase-3/7 Z-DEVD-R110 Assay Kit provides a method for detecting apoptosis by providing a simple and reliable method for assaying caspase-3/7 activity. The kit can be used to continuously measure the activity of caspase-3/7 in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader. The rhodamine […]
Continue Reading
Caspase Activity Assays
Introduction To Order Related Products Introduction A distinctive feature of the early stages of apoptosis is the activation of caspase enzymes, which participate in the cleavage of protein substrates and in the subsequent disassembly of the cell. ABP Biosciences offer a series of caspase assays that allow the simple detection of active caspases in living cells in […]
Continue Reading 5X Annexin V Binding Buffer
Introduction To order Documents Related products Introduction This product is provided as concentrated buffer to facilitate the binding of annexin V to phosphatidylserine for use in apoptosis assays. Formulation: 50 mM HEPES, 700 mM NaCl, 12.5 mM CaCl2, pH 7.4. To order Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price A041 […]
Continue Reading Andy Fluor™ 680 Annexin V
Introduction To order Documents Related products Introduction Andy Fluor™ 680 Annexin V is Annexin V conjugated with our far-red fluorescent dye Andy Fluor™ 680. Annexin V is a calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, […]
Continue ReadingCopyright@ 2025 ABP Biosciences, LLC. All Rights Reserved.
