eGFP Annexin V and PI Apoptosis Kit

Introduction

Our eGFP Annexin V and PI Apoptosis Kit is used to detect the externalization of phosphatidylserine in apoptotic cells. This kit provides a sensitive two-color assay that employs eGFP-annexin V and a ready-to-use solution of the red-fluorescent propidium iodide nucleic acid stain. Propidium iodide is impermeant to live cells and apoptotic cells but stains necrotic cells with red fluorescence, binding tightly to the nucleic acids in the cell. After staining a cell population with eGFP-annexin V and propidium iodide in the provided binding buffer, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished using a flow cytometer with the 488 nm spectral line of an argon-ion laser for excitation.

Specifications

Platform:	Fluorescence Cytometer
Detection Method:	Fluorescent
Ex/Em:	PI: 535/617 nm; eGFP: 475/509 nm
Product Size:	100 assays
Storage Conditions:	4 ℃, protect from light
Shipping Condition:	Ice pack
Applications Cell apoptosis analysis.

Figure 1. Jurkat cells treated with 10 μM camptothecin for four hours (right panel) or untreated (as control, left panel). Cells were then treated with the reagents in the kit, followed by flow cytometric analysis.

To Order

Documents

Protocol (PDF):	A025
MSDS (PDF):	Component A Component B Component C
COA (PDF):	A025

Cited Reference:

Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
Sema Serter Kocoglu, Mücahit Seçme, Levent Elmas
Arch Biol Sci. 2022;74(3):227-234; https://doi.org/10.2298/ABS220219021S

Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
Serter Koçolu S, Seçme M, Elmas L.
Arch Biol Sci 2022, 74(3):227-34. https://doi.org/10.2298/ABS220219021S

Copper Nanoparticles Induce Oxidative Stress via the Heme Oxygenase 1 Signaling Pathway in vitro Studies
Zou L, Cheng G, Xu C, Liu H, Wang Y, Li N, Fan X, Zhu C, Xia W
Int J Nanomedicine. 2021, 16:1565-1573. doi: 10.2147/IJN.S292319

Activation of Aquaporin 5 by carcinogenic Helicobacter pylori infection promotes epithelial-mesenchymal transition via the MEK/ERK pathway
Li, N., Xu, X., Yang, H., Wang, H., Ouyang, Y., Zhou, Y., … Hong, J.
Helicobacter. 2021,00:e12842. https://doi.org/10.1111/hel.12842

Fresh and cultured mouse islets differ in their response to nutrient stimulation
Morsi M, Schulze T, Früh E, Brüning D, Panten U, Rustenbeck I.
Endocr Connect. 2020, 9(8):769-782. doi: 10.1530/EC-20-0289

Naringin induces endoplasmic reticulum stress-mediated apoptosis, inhibits catenin pathway and arrests cell cycle in cervical cancer cells
Ruyin Lin, Xinxin Hu, Shaorong Chen, Qiyang Shi and Huiqing Chen
Acta Biochimica Polonica, 2020, 67, 181-188. https://doi.org/10.18388/abp.2020_5182

Pharmacological inhibition of thioredoxin reductase increases insulin secretion and diminishes beta cell viability
Brüning, D., Hatlapatka, K., Lier-Glaubitz, V. et al.
Naunyn-Schmiedeberg's Arch Pharmacol 394, 1133–1142 (2021). https://doi.org/10.1007/s00210-020-02046-2

MicroRNA function can be reversed by altering target gene expression levels
Alexander A. Svoronos, Stuart G. Campbell, Donald M. Engelman
iScience, 2021, 24, 10, 103208, https://doi.org/10.1016/j.isci.2021.103208

Monensinin Glioblastoma Multiformede Kaspaz-10 Aracılı Apoptoz Üzerine Etkileri
Sema SERTER KOÇOLU, Mücahit SEÇME
Uluda Üniversitesi Tıp Fakültesi Dergisi, 47 (3) 393-397, 2021. DOI: https://doi.org/10.32708/uutfd.999511

[11]-Chaetoglobosins with Cytotoxic Activities from Pseudeurotium Bakeri
Ruan, Hanli and Duan, Fangfang and Gao, Ying and Peng, Xiaogang and Meng, Xianggao and Chang, Jinling and Gan, Yutian and Ouyang, Qianxi
SSRN: http://dx.doi.org/10.2139/ssrn.4102564

Related Products

Buy	Cat.No.	Product Name	Ex/Em	Unit	Price
	A025	eGFP Annexin V and PI Apoptosis Kit	475/509	100 assays	$200