• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on CFDA

Introduction To Order Documents Related Products Introduction 5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-carboxyfluorescein diacetate yields 5(6)-carboxyfluorescein. […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on BCECF, AM

Introduction To Order Documents Related Products Introduction BCECF, AM is an ideal cell-permeant, dual-excitation ratiometric pH indicator for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on BCECF

Introduction To Order Documents Related Products Introduction BCECF is an ideal dual-excitation ratiometric pH indicator for measuring changes ± 1 pH unit around pH 7 with a pKa of ~6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on Calcein, AM

Introduction To Order Documents Related Products Introduction Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein, after acetoxymethyl ester hydrolysis by intracellular esterases. This dye is provided as 1 mg solid (C025) and […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on Calcein blue, AM

Introduction To Order Documents Related Products Introduction Calcein Blue, AM is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Calcein blue AM is only weakly fluorescent (excitation/emission maxima ~322/435 nm). Upon cleavage of the […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on Pluronic F-127®

Introduction To Order Documents Related Products Introduction Pluronic F-127 is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of ion indicators as well as the […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on DiR iodide

Introduction To Order Documents Related Products Introduction DiR is a near IR fluorescent, lipophilic carbocyanine and widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on DiD perchlorate

Introduction To Order Documents Related Products Introduction DiD is a far-red fluorescent, lipophilic carbocyanine and widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent when incorporated into membranes.  It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on DiI perchlorate

Introduction To Order Documents Related Products Introduction DiI is an orange-red fluorescent, lipophilic carbocyanine and widely used as a long-term tracer for neuronal and other cells. It is weakly fluorescent in water but highly fluorescent when incorporated into membranes. Once applied to cells, the dye diffuses laterally to stain the entire cell.    Specifications:   […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on DiO perchlorate

Introduction To Order Documents Related Products Introduction DiO is a green fluorescent, lipophilic carbocyanine and widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied […]