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Tracking New DNA Synthesis

Introduction

Measuring the synthesis of new DNA is a precise way to assay cell proliferation in individual cells or in cell populations. Our iClick™ EdU assays are designed to analyze the rate of new DNA synthesis based on incorporation of the nucleoside analog EdU into DNA. Detection is achieved through a copper-catalyzed “click” reaction that is complete typically within 30 minutes. The iClick™ EdU assays have great advantage compared to the traditional BrdU assay (Figure 1). Our iClick™ EdU assays use mild reaction conditions, and our bright Andy Fluor™ 'click' labels offer multicolor choice for multiplexing. The assay kits can be used for cultured cells or tissue sections (Figure 2).

Figure 1. Detection of new DNA synthesis with EdU or BrdU. The small size of Andy Fluor™ azide can easily access to incorporated EdU for 'click' reaction without the need to denature the DNA. Without DNA denaturation, incorporated BrdU is inaccessible to antibodies used for detection.

Figure 2. Detection of cell proliferation in cell and mouse tissue. A549 cells were treated with 10 μM EdU for 2 hr, then detected with Andy Fluor™ 488 azide (green, top), and Andy Fluor™ 594 azide (red, middle), cells were counterstained with DAPI (blue). A mouse was injected intraperitoneally with 5 mg EdU per kilogram body weight, then sacrificed at 0, 12, and 24 hr. The mouse intestine tissue was detected with Andy Fluor™ 488 azide (green, bottom), and counterstained with DAPI (blue).

Selection Guide for iClick™ EdU Cell Proliferation Kit

Cat. No. Product Name Application Ex/Em (nm) Unit
A003 iClick™ EdU Andy Fluor™ 488 Imaging Kit Imaging 495/520 50 assays
A004 iClick™ EdU Andy Fluor™ 555 Imaging Kit Imaging 555/565 50 assays
A005 iClick™ EdU Andy Fluor™ 594 Imaging Kit Imaging 590/615 50 assays
A006 iClick™ EdU Andy Fluor™ 647 Imaging Kit Imaging 650/665 50 assays
A007 iClick™ EdU Andy Fluor™ 488 Flow Cytometry Assay Kit Flow Cytometry 495/520 50 assays
A008 iClick™ EdU Andy Fluor™ 647 Flow Cytometry Assay Kit Flow Cytometry 650/665 50 assays
A011 BrdU     50 mg
A012 EdU     50 mg
A013 EU     50 mg

To order

Buy Cat.No. Product Name Application Ex/Em Unit Price
A003 iClick™ EdU Andy Fluor™ 488 Imaging Kit Imaging 495/520 50 assays $320
A004 iClick™ EdU Andy Fluor™ 555 Imaging Kit Imaging 555/565 50 assays $320
A005 iClick™ EdU Andy Fluor™ 594 Imaging Kit Imaging 590/615 50 assays $320
A006 iClick™ EdU Andy Fluor™ 647 Imaging Kit Imaging 650/665 50 assays $320
A007 iClick™ EdU Andy Fluor™ 488 Flow Cytometry Assay Kit Flow Cytometry 495/520 50 assays $350
A008 iClick™ EdU Andy Fluor™ 647 Flow Cytometry Assay Kit Flow Cytometry 650/665 50 assays $350
A011 BrdU     50 mg $80
A012 EdU     50 mg $80
A013 EU     50 mg $80