Detection of newly synthesized RNA is an important method to measure cell proliferation and toxicological profiling. Our iClick™ EU assays utilize an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and powerful click chemistry to detect newly synthesized RNA in a simple, two-step procedure. In step one, the alkyne-containing nucleoside is fed to cells or animals and actively incorporated into nascent RNA. Detection utilizes the“click" reaction between an azide and an alkyne where the modified RNA is detected with a corresponding azide-containing dye (Figure 1).
Figure 1. Detection of cell proliferation in cell and mouse tissue. A549 cells were treated with 100 μM EU for 2 hr, then detected with Andy Fluor™ 488 azide (green, top), and Andy Fluor™ 594 azide (red, middle), cells were counterstained with DAPI (blue). A mouse was injected intraperitoneally with 50 mg EU per kilogram body weight, then sacrificed at 0, 12, and 24 hr. The mouse intestine tissue was detected with Andy Fluor™ 488 azide (green, bottom), and counterstained with DAPI (blue).
Selection Guide for iClick™ EU Cell Proliferation Kit
|Cat. No.||Product Name||Application||Ex/Em||Unit|
|A009||iClick™ EU Andy Fluor™ 488 Imaging Kit||Imaging||495/520||50 assays|
|A010||iClick™ EU Andy Fluor™ 594 Imaging Kit||Imaging||590/615||50 assays|