Introduction
Fluo-4, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-4 becomes fluorescent in the presence of free Ca2+. Fluo-4 AM is widely used in flow cytometry, confocal laser scanning microscopy and fluorescence spectroscopy. Fluo-4, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening.
Fluo-4, AM is supplied as 0.5 mg solid (C219), special packing 10×50 μg (C220), and 1 mg/ml solution in DMSO (C221).
Specifications
Label:
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Fluo-4
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Ex/Em:
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494/506
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Detection Method:
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Fluorescent
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Solubility:
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DMSO, DMF
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Molecular Formula:
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C51H50F2N2O23
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Molecular Weight:
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1096.95
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CAS Number:
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273221-67-3
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Storage Conditions:
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-20 ℃, protect from light
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Shipping Condition:
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Room Temperature
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Applications
Calcium indicator
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Documents
References:
Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo.
Garaschuk O, Milos RI, Konnerth A
Nat Protoc (2006) 1:380-386
Local potassium signaling couples neuronal activity to vasodilation in the brain.
Filosa JA, Bonev AD, Straub SV, Meredith AL, Wilkerson MK, Aldrich RW, Nelson MT
Nat Neurosci (2006) 9:1397-1403
Negative regulation of Fc epsilonRI-mediated signaling and mast cell function by the adaptor protein LAX.
Zhu M, Rhee I, Liu Y, Zhang W
J Biol Chem (2006) 281:18408-18413