Difluorocarboxyfluorescein, succinimidyl ester, 5-isomer

Introduction

The amine-reactive difluorocarboxyfluorescein, succinimidyl ester, 5-isomer can be used to create green fluorescent bioconjugates with excitation/emission maxima ~496/524 nm. This fluorinated analog of fluorescein overcomes some of the key limitations of fluorescein, including greater photostability and a lower pK_a (pK_a ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.

Specifications

Label:	Difluorofluorescein
Ex/Em:	496/524 nm
Detection Method:	Fluorescent
Solubility:	DMSO, DMF
Molecular Formula:	C₂₅H₁₃F₂NO₉
Molecular Weight:	509.38
CAS Number:	198139-51-4
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications Fluorescent labeling

To order

Documents

Datasheet (PDF):	C114
MSDS (PDF):	MSDS-C114

References:

A comparison of the emission efficiency of four common green fluorescence dyes after internalization into cancer cells.
Hama Y, Urano Y, Koyama Y, Bernardo M, Choyke PL, Kobayashi H
Bioconjug Chem (2006) 17:1426-1431.

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C114	5-OG 488, SE	496/524	5 mg	$165