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AMF [4′-(Aminomethyl)fluorescein]

Introduction

The primary aliphatic amine of 4'-(aminomethyl)fluorescein can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNBH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC.
 

Specifications

Label: Fluorescein
Ex/Em: 494/520 nm
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C21H16ClNO5
Molecular Weight: 397.81
CAS Number: 91539-64-9
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Fluorescent labeling

To order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C301 4'-(Aminomethyl)fluorescein 494/520 25 mg $95

Documents

Datasheet (PDF): C301
MSDS (PDF): MSDS-C301

References:

Swelling-based method for preparing stable, functionalized polymer colloids.
Kim AJ, Manoharan VN, Crocker JC
J Am Chem Soc (2005) 127:1592-1593

4′-[Aminomethyl]fluorescein and its N-alkyl derivatives: useful reagents in immunodiagnostic techniques.
Shipchandler MT, Fino JR, Klein LD, Kirkemo CL
Anal Biochem (1987) 162:89-101

Redesigning an FKBP-ligand interface to generate chemical dimerizers with novel specificity.
Clackson T, Yang W, Rozamus LW, Hatada M, Amara JF, Rollins CT, Stevenson LF, Magari SR, Wood SA, Courage NL, Lu X, Cerasoli F, Gilman M, Holt DA
Proc Natl Acad Sci U S A (1998) 95:10437-10442