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Tyramide Signal Amplification (TSA)


Tyramide signal amplification (TSA) is a highly sensitive method enabling the detection of low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (FISH) applications.

Tyramide signal amplification (TSA) process involves the use of horseradish peroxidase (HRP) to catalyze the deposition of fluorophore tyramides on and near a target protein or nucleic acid sequence in situ. In the presence of low concentrations of H2O2, HRP is able to convert a labeled tyramide substrate into a highly reactive form that can covalently bind to tyrosine residues on proteins at or near the HRP. This generates high density tyramide labeling and is the reason for the exceptional sensitivity of this system. 


  • Highly sensitive detection of low-abundance, hard-to-detect targets
  • Compatible with ICC, IHC and FISH
  • High-resolution and multiplex imaging
  • Similar workflow to conventional ICC, IHC and FISH

Figure 1. Illustration of the tyramide signal amplification system.

Figure 2. Multiplex tyramide labeling of FFPE tissues. 


To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
L040 Andy Fluor 488 Tyramide 505/526 0.5 µmol $260
L041 Andy Fluor 555 Tyramide 553/565 0.5 µmol $260
L042 Andy Fluor 594 Tyramide 590/615 0.5 µmol $260
L043 Andy Fluor 647 Tyramide 650/666 0.5 µmol $260
L152A HRP Goat Anti-Mouse IgG (H+L) Antibody   100 µg $40
L152B HRP Goat Anti-Mouse IgG (H+L) Antibody   500 µg $80
L153A HRP Goat Anti-Rabbit IgG (H+L) Antibody   100 µg $40
L153B HRP Goat Anti-Rabbit IgG (H+L) Antibody   500 µg $80