Tyramide Signal Amplification (TSA)

Introduction

Tyramide signal amplification (TSA) is a highly sensitive method enabling the detection of low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (FISH) applications.

Tyramide signal amplification (TSA) process involves the use of horseradish peroxidase (HRP) to catalyze the deposition of fluorophore tyramides on and near a target protein or nucleic acid sequence in situ. In the presence of low concentrations of H₂O₂, HRP is able to convert a labeled tyramide substrate into a highly reactive form that can covalently bind to tyrosine residues on proteins at or near the HRP. This generates high density tyramide labeling and is the reason for the exceptional sensitivity of this system.

Features

Highly sensitive detection of low-abundance, hard-to-detect targets
Compatible with ICC, IHC and FISH
High-resolution and multiplex imaging
Similar workflow to conventional ICC, IHC and FISH

Figure 1. Illustration of the tyramide signal amplification system.

Figure 2. Multiplex tyramide labeling of FFPE tissues.

To order

Buy	Cat.No.	Product Name	Ex/Em(nm)	Unit	Price
	L040	Andy Fluor 488 Tyramide	505/526	0.5 µmol	$260
	L041	Andy Fluor 555 Tyramide	553/565	0.5 µmol	$260
	L042	Andy Fluor 594 Tyramide	590/615	0.5 µmol	$260
	L043	Andy Fluor 647 Tyramide	650/666	0.5 µmol	$260
	L152	HRP Goat Anti-Mouse IgG (H+L) Antibody		100 µL	$50
	L153	HRP Goat Anti-Rabbit IgG (H+L) Antibody		100 µL	$50

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