The SafeGreen™ Loading Dye is used for running DNA samples on agarose gel. The SafeGreen™ Loading Dye contains two regular dyes (Xylene Cyanol FF and Bromophenol Blue), and a safe, non-carcinogen green fluorescent dye. The Xylene Cyanol FF and Bromophenol Blue are used for monitoring the agarose gel while it is running, and the green fluorescent dye is to replace the toxic carcinogen ethidium bromide (EtBr). During the gel electrophoresis, the agarose gel will reveal a purple band and a blue band. The gel can be directly visualized under LED or UV light without any extra staining steps.