The ABP Renilla Luciferase Assay Kit is a single-addition reagent that generates a glow-type signal with Renilla luciferase. When reconstituted, it has the capacity to lyse cells, reduce the autoluminescence of the coelenterazine substrate, and produce a stable signal (half-life greater than 60 minutes at 22°C).
The ABP Renilla Luciferase Assay System is suitable for use in high-throughput screening assays. The "add-and-read" method simplifies assay optimization, and there is no need for separate lysis and reagent injection steps. The extended, bright signal is optimized for performance in either batch or continuous process handling.
- No centrifugation or pre-lysis steps required. Reagents are added directly to growth medium
- Stable signal allows flexibility for batch or continuous processing of 96- and 384-well plates
- Simple "add-and-read" protocol is compatible with any luminometer
- Lower background, higher sensitivity
Figure 1. The Renilla luciferase reaction