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H2DCFDA [2′,7′-Dichlorodihydrofluorescein diacetate]


H2DCFDA [2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent H2DCFDA is converted to the highly fluorescent 2',7'-dichlorofluorescein.


Label: 2',7'-Dichlorofluorescein
Ex/Em: 495/529 nm
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C24H16Cl2O7
Molecular Weight: 487.29
CAS Number: 4091-99-0
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Probe for ROS

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C263 H2DCFDA 495/529 50 mg $88


Datasheet (PDF): C263


Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.
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PLoS One (2012) 7:e35399-e35399

Complex N-glycan and metabolic control in tumor cells.
Mendelsohn R, Cheung P, Berger L, Partridge E, Lau K, Datti A, Pawling J, Dennis JW,
Cancer Res (2007) 67:9771-9780

Detecting enzymatic activity in cells using fluorogenic substrates.
Haugland RP
Biotech Histochem (1995) 70:243-251

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