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DHR 123 [Dihydrorhodamine 123]


Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide and peroxynitrite. 


Label: Rhodamine 123
Ex/Em: 507/529 nm
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C21H18N2O3
Molecular Weight: 346.38
CAS Number: 109244-58-8
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Probe for ROS

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Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C261 DHR 123 507/529 10 mg $165


Datasheet (PDF): C261


Superoxide reacts with nitric oxide to nitrate tyrosine at physiological pH via peroxynitrite.
Reiter CD, Teng RJ, Beckman JS
J Biol Chem (2000) 275:32460-32466

Reaction of superoxide and nitric oxide with peroxynitrite. Implications for peroxynitrite-mediated oxidation reactions in vivo.
Jourd'heuil D, Jourd'heuil FL, Kutchukian PS, Musah RA, Wink DA, Grisham MB
J Biol Chem (2001) 276:28799-28805

Modulation of superoxide-dependent oxidation and hydroxylation reactions by nitric oxide.
Miles AM, Bohle DS, Glassbrenner PA, Hansert B, Wink DA, Grisham MB
J Biol Chem (1996) 271:40-47

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