H2DCFDA [2′,7′-Dichlorodihydrofluorescein diacetate]

Introduction

H₂DCFDA [2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent H₂DCFDA is converted to the highly fluorescent 2',7'-dichlorofluorescein.

Specifications

Label:	2',7'-Dichlorofluorescein
Ex/Em:	495/529 nm
Detection Method:	Fluorescent
Solubility:	DMSO, DMF
Molecular Formula:	C₂₄H₁₆Cl₂O₇
Molecular Weight:	487.29
CAS Number:	4091-99-0
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications Probe for ROS

To order

Documents

Datasheet (PDF):	C263
MSDS (PDF):	MSDS-C263

References:

Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.

Kim YC, Kim BG, Lee JH,
PLoS One (2012) 7:e35399-e35399

Complex N-glycan and metabolic control in tumor cells.

Mendelsohn R, Cheung P, Berger L, Partridge E, Lau K, Datti A, Pawling J, Dennis JW,
Cancer Res (2007) 67:9771-9780

Detecting enzymatic activity in cells using fluorogenic substrates.

Haugland RP
Biotech Histochem (1995) 70:243-251

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Buy	Cat.No.	Product Name	Ex/Em(nm)	Unit	Price
	C263	H₂DCFDA	495/529	50 mg	$88

H2DCFDA [2′,7′-Dichlorodihydrofluorescein diacetate]

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