EasySC Viral RNA Mini Kit

EasySC Viral RNA Mini Kit is one of the reagents used to isolate viral RNA from respiratory samples and is currently also used to detect coronavirus SARS-CoV-2, which causes COVID-19.

EasySC Viral RNA Mini Kit is designed for the fastest and easiest way to purify viral RNA from serum, plasma, swab, urine, cell culture media, and other cell-free body fluids. The EasySC Viral RNA Mini Kit can be fully automated on Qiagen QIAcube Connect. The sample is first lysed under highly denaturing conditions to inactivate RNase and to ensure isolation of intact viral RNA. Buffering conditions are then adjusted to provide optimum binding of the RNA to the spin column membrane, and the sample is loaded onto the Mini spin column. The RNA binds to the membrane, and contaminants are efficiently washed away using washing buffers. High-quality RNA is eluted for direct use or safe storage. The purified RNA is free of protein, nucleases and other contaminants and inhibitors, and is ready for downstream applications such as Next-Gen sequencing, and RT-qPCR detection.

Features

• High sensitivity: the kit can be used to isolate viral RNA as little as 10 copies of virus.
• Fast: the purification process takes only 20 minutes.
• Good stability: the optimized buffer system provides consistent results.
• Universal: the kit is compatible with a wide variety of viruses.

Figure 1. RT-qPCR results of purified RNA using EasySC Viral RNA Mini Kit.
Samples: Total RNA extracted from Bird Flu Standard (standard diluted by 20, 40, 80, 160, 320, 640, 1280, 2560, 5120).
Primers & Probes: Bird Flu Virus specific primers and probes.