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EasyMag Viral RNA Mini Kit

Introduction

EasyMag Viral RNA Mini Kit is one of the reagents used to isolate viral RNA from respiratory samples and is currently also used to detect coronavirus SARS-CoV-2, which causes COVID-19.

EasyMag Viral RNA Mini Kit is designed for the fastest and easiest way to purify viral RNA from serum, plasma, swab, urine, cell culture media, and other cell-free body fluids. The magnetic beads technology enables purification of high-quality RNA that is free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can be also be scaled up or down depending on the amount of starting sample. The sample is first lysed under highly denaturing conditions to inactivate RNase and to ensure isolation of intact viral RNA. Buffering conditions are then adjusted to provide optimum binding of the RNA to the magnetic beads. The RNA binds to the magnetic beads, and contaminants are efficiently washed away using washing buffers. High-quality RNA is then eluted from magnetic beads for direct use or safe storage. The purified RNA is ready for downstream applications such as Next-Gen sequencing, and RT-qPCR detection.


Features

  • High sensitivity: the kit can be used to isolate viral DNA/RNA as little as 10 copies of virus.
  • Fast: the purification process takes only 30 minutes.
  • Good stability: the optimized buffer system provides consistent results.
  • Universal: the kit is compatible with various liquid samples.

 

Technical Specifications

Applications Viral RNA from serum, plasma, swab, urine, cell culture media, and other cell-free body fluids.
Sample Source Serum, plasma, swab, urine, cell culture media, and other cell-free body fluids.
Sample Input 140 μL body fluids
Elution Volume 60 μL
Technology Magnetic Beads
Processing Mode Manual, Automated

 
 

  ABP Company A  
Sample 1 29.08 29.04 32.15 32.50
29.13 32.41
28.92 32.95

Table 1. RT-qPCR results of purified HCV RNA using ABP EasyMag Viral RNA Mini Kit and Company A kit.

To Order

Buy Cat.No. Product Name Unit Size Price
D144-1 EasyMag Viral RNA Mini Kit 50 preps $180
D144-2 EasyMag Viral RNA Mini Kit 200 preps $560

Documents

Protocol (PDF):

D144

MSDS (PDF): 

Buffer AVL                Buffer AW1

Buffer AW2               Carrier RNA