EasySC Gel DNA Extraction Kit uses the proprietary spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 80%). The kit uses a specialized binding buffer system that dissolves the gel slice and binds to the spin column. The bind step is followed by three rapid wash steps and DNA is eluted with elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.
Technical Specifications
Figure 1. 5 different size DNA fragments were recovered using ABP EasySC Gel DNA Extraction Kit. DNA was analyzed on a 1% TBE agarose gel.
Features
Applications
DNA isolation from agarose gel.
Sample Source
Agarose gel slice
Sample Input
Up to 20 μg DNA
Elution Volume
30-50 μL
Technology
Silica spin column
Processing Mode
Manual, Centrifugation/Vacuum
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