Introduction
EasyProbes™ ActinGreen 488 Stain is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Andy Fluor™ 488 dye. Our EasyProbes™ ActinGreen 488 Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops of stain solution to the cell slide to stain your cells.
Features
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High-affinity staining of F-actin with superior specificity compared to antibody methods
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Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
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Bright fluorescence signal
Suggestions for use
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In most cases 2 drops of stain solution and an incubation of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
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EasyProbes™ ActinGreen 488 Stain is excited with a maximum at 500 nm, with an emission maximum at 525 nm. It is detected through standard GFP and FITC filters.
Specifications:
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Excitation/Emission:
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500/525 nm
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Shipping Condition:
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Ambient
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Storage Conditions:
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4ºC, protect from light
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Unit Size:
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5 mL
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Documents
Reference:
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J Cell Biol (2012) 199:425-435
Drug-selected human lung cancer stem cells: cytokine network, tumorigenic and metastatic properties.
Levina V, Marrangoni AM, DeMarco R, Gorelik E, Lokshin AE,
PLoS One (2008) 3:e3077-e3077
Expression levels of a kinesin-13 microtubule depolymerase modulates the effectiveness of anti-microtubule agents.
Schimizzi GV, Currie JD, Rogers SL,
PLoS One (2010) 5:e11381-e11381