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Cy5 Azide

Introduction

Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
The Cy5 azide is reactive with terminal alkyne via a copper-catalyzed click reaction that allows the subsequent visualization by fluorescence spectroscopy.
 

Features

  • Efficiency—the click reaction is complete in less than 1 hour;
  • Specificity—the reaction between the label and detection tag is selective and specific;
  • Stability—the reaction product contains an irreversible, covalent bond;
  • Biologically inert—the components of the reaction do not undergo any side reactions.


 
Figure 1. Click chemistry labeling


Specifications

Label: Cy5
Ex/Em: 650/665
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Weight: 900.16
Product Size: 1 µmol
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Click chemistry labeling

 

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C314 Cy5 Azide 650/665 1 µmol $180
 

Documents

Datasheet (PDF): C314
MSDS (PDF): C314

Reference

Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,
Angew Chem Int Ed Engl (2012) 51:3143-3146

Winz ML, Samanta A, Benzinger D, Jäschke A,
Nucleic Acids Res (2012) 40:e78-e78

Ngo JT, Schuman EM, Tirrell DA,
Proc Natl Acad Sci U S A (2013) 110:4992-4997
Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells.
Hsu TL, Hanson SR, Kishikawa K, Wang SK, Sawa M, Wong CH
Proc Natl Acad Sci U S A (2007) 104:2614-2619

Metabolic labeling of sialic acids in living animals with alkynyl sugars.
Chang PV, Chen X, Smyrniotis C, Xenakis A, Hu T, Bertozzi CR, Wu P
Angew Chem Int Ed Engl (2009) 48:4030-4033