The primary aliphatic amine of 4'-(aminomethyl)fluorescein can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNBH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC.
Specifications
Fluorescent labeling
References:
Label:
Fluorescein
Ex/Em:
494/520 nm
Detection Method:
Fluorescent
Solubility:
DMSO, DMF
Molecular Formula:
C21H16ClNO5
Molecular Weight:
397.81
CAS Number:
91539-64-9
Storage Conditions:
-20 ℃, protect from light
Shipping Condition:
Room Temperature
Applications
Datasheet (PDF):
C301
MSDS (PDF):
MSDS-C301
J Am Chem Soc (2005) 127:1592-1593
Anal Biochem (1987) 162:89-101
Proc Natl Acad Sci U S A (1998) 95:10437-10442
AMF [4′-(Aminomethyl)fluorescein]
Introduction
To order
Documents
Kim AJ, Manoharan VN, Crocker JC
Shipchandler MT, Fino JR, Klein LD, Kirkemo CL
Clackson T, Yang W, Rozamus LW, Hatada M, Amara JF, Rollins CT, Stevenson LF, Magari SR, Wood SA, Courage NL, Lu X, Cerasoli F, Gilman M, Holt DA
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