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5-Ethynyl-dUTP (5-EdUTP)


5-Ethynyl-dUTP (5-EdUTP) (cat. no. C432) is supplied as a 1 mM solution in TE buffer.

5-EdUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.

The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice

  • to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
  • to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
  • to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins


Concentration: 1 mM solution in TE buffer
Storage: Store at -20°C
Molecular Formula: C11H15N2O14P3 (free acid)
Molecular Weight: 492.16 (free acid)



Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension.


To order

Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price
C432 5-EdUTP, 1 mM in TE buffer 500 µL $200




Protocol (PDF): C432