DAPI I Counterstain | ABP Biosciences

Introduction

DAPI I Counterstain is used as a counterstain for chromosomes. The counterstain consists of DAPI (1500 ng/ml) and antifade, which helps preserving the fluorescence signal. DAPI binds strongly to DNA, particularly to AT rich regions. It is ideal for use with FISH and other fluorescence assays.

Key Features:

Stains nuclei with sharp, blue fluorescence.
Built-in antifade reagent extends signal visibility.
Ready-to-use formulation for easy workflow integration.
Ideal for use with FISH and other fluorescence assays.

To Order

Documents

Protocol (PDF):	C002-1
MSDS (PDF):	MSDS-C002-1

Reference:

An automated image capture and quantitation approach to identify proteins affecting tumor cell proliferation.

Bhawe KM, Blake RA, Clary DO, Flanagan PM
J Biomol Screen (2004) 9:216-222

Detection of pyruvate dehydrogenase E1 alpha-subunit deficiencies in females by immunohistochemical demonstration of mosaicism in cultured fibroblasts.

Lib MY, Brown RM, Brown GK, Marusich MF, Capaldi RA
J Histochem Cytochem (2002) 50:877-884

Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission.

Dagda RK, Cherra SJ, Kulich SM, Tandon A, Park D, Chu CT,
J Biol Chem (2009) 284:13843-13855

Frequently Asked Questions (FAQs)

Is DAPI a good live-cell nuclear label?
DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label non-uniformly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

My DAPI labeled samples have a strong blue background signal immediately after mounting. What can I do to fix this?
Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.

Related Products

Buy	Cat.No.	Product name	Ex/Em (nm)	Size	Price
	C002-1	DAPI I Counterstain	358/461	2×500 µL	$45