EasySC Plasmid Mini Purification Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with Spin Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
Features
- Rapid – Purification of plasmid DNA in less than 30 minutes.
- Safe – No Phenol/chloroform extractions.
- High-quality – DNA is suitable for a variety of downstream applications.
Technical Specifications |
|
Applications | Plasimd DNA isolation from 1-6 mL bacterial cultures. |
Sample Source | LB culture |
Sample Input | 1-6 mL high-copy plasimd, 10 mL low-copy plasimd |
Elution Volume | 30-100 μL |
Technology | Silica spin column |
Processing Mode | Manual, Centrifugation/Vacuum |
Figure 1. 5 µL of purified pUC18 and pUC19 plasmids were analyzed on a 1% Agarose gel. Plasmid was isolated from 5 mL DH5α cultures harboring the plasmid using ABP EasySC Plasmid Mini Purification Kit.
Table 1. DNA yield and quality using ABP EasySC Plasmid Mini Purification Kit
Protocol (PDF): |
|
MSDS (PDF): |
Related Products
- Genomic DNA Purification
- RNA Purification
- Viral DNA & RNA Purification
- Plasmid DNA Purification
- PCR & Gel Cleanup
- Spin Column & Magnetic Beads
- DNA & RNA Gel Electrophoresis
- DNA & RNA Quantitation