EasySC Plasmid Mini Purification Kit

Introduction

EasySC Plasmid Mini Purification Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with Spin Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.

Features

Rapid – Purification of plasmid DNA in less than 30 minutes.
Safe – No Phenol/chloroform extractions.
High-quality – DNA is suitable for a variety of downstream applications.

Technical Specifications
Applications	Plasimd DNA isolation from 1-6 mL bacterial cultures.
Sample Source	LB culture
Sample Input	1-6 mL high-copy plasimd, 10 mL low-copy plasimd
Elution Volume	30-100 μL
Technology	Silica spin column
Processing Mode	Manual, Centrifugation/Vacuum

Figure 1. 5 µL of purified pUC18 and pUC19 plasmids were analyzed on a 1% Agarose gel. Plasmid was isolated from 5 mL DH5α cultures harboring the plasmid using ABP EasySC Plasmid Mini Purification Kit.

Table 1. DNA yield and quality using ABP EasySC Plasmid Mini Purification Kit