EasyProbe™ ActinRed 555 Stain

Introduction

EasyProbes™ ActinRed 555 Stain is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TMR). Our EasyProbes™ ActinRed 555 Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops of stain solution to the cell slide to stain your cells.

Features

High-affinity staining of F-actin with superior specificity compared to antibody methods
Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
Bright fluorescence signal

Suggestions for use

In most cases 2 drops of stain solution and an incubation of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
EasyProbes™ ActinRed 555 Stain is excited with a maximum at 540 nm, with an emission maximum at 565 nm. It is detected through standard standard TRITC filter.

Specifications:
Excitation/Emission:	555/575 nm
Shipping Condition:	Ambient
Storage Conditions:	4ºC, protect from light
Unit Size:	5 mL

To Order

Documents

Protocol (PDF):	FP032
MSDS (PDF):	FP032
COA (PDF):	FP032

Reference:

Fabrication of high specificity hollow mesoporous silica nanoparticles assisted by Eudragit for targeted drug delivery.

She X, Chen L, Velleman L, Li C, Zhu H, He C, Wang T, Shigdar S, Duan W, Kong L,
J Colloid Interface Sci (2015) 445:151-160

In situ measurement of magnetization relaxation of internalized nanoparticles in live cells.

Soukup D, Moise S, Céspedes E, Dobson J, Telling ND,
ACS Nano (2015) 9:231-240

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	FP032	EasyProbe™ ActinRed 555 Stain	555/575	5 mL	$165