EasyProbe™ Actin 488 Stain

Introduction

EasyProbes™ Actin 488 Stain is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Andy Fluor™ 488 dye. Our EasyProbes™ Actin 488 Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops of stain solution to the cell slide to stain your cells.

Features

High-affinity staining of F-actin with superior specificity compared to antibody methods
Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
Bright fluorescence signal

Suggestions for use

In most cases 2 drops of stain solution and an incubation of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
EasyProbes™ Actin 488 Stain is excited with a maximum at 500 nm, with an emission maximum at 525 nm. It is detected through standard GFP and FITC filters.

Specifications:
Excitation/Emission:	500/525 nm
Shipping Condition:	Ambient
Storage Conditions:	4ºC, protect from light
Unit Size:	5 mL

To Order

Documents

Protocol (PDF):	FP031
MSDS (PDF):	FP031
COA (PDF):	FP031

Reference:

Phosphocaveolin-1 is a mechanotransducer that induces caveola biogenesis via Egr1 transcriptional regulation.

Joshi B, Bastiani M, Strugnell SS, Boscher C, Parton RG, Nabi IR,
J Cell Biol (2012) 199:425-435

Drug-selected human lung cancer stem cells: cytokine network, tumorigenic and metastatic properties.

Levina V, Marrangoni AM, DeMarco R, Gorelik E, Lokshin AE,
PLoS One (2008) 3:e3077-e3077

Expression levels of a kinesin-13 microtubule depolymerase modulates the effectiveness of anti-microtubule agents.

Schimizzi GV, Currie JD, Rogers SL,
PLoS One (2010) 5:e11381-e11381

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	FP031	EasyProbe™ Actin 488 Stain	500/525	5 mL	$165