Hoechst 33342 is a popular cell membrane-permeant nuclear counterstain. Hoechst 33342 emits blue fluorescence upon binding to the minor groove of dsDNA. Hoechst 33342 are widely used for nuclear counterstaining, apoptosis and cell cycle studies. Hoechst 33342 is provided as a 10 mg solid (C005) and a 10 mg/mL aqueous solution (C006).
| Specifications: | |
| Excitation/Emission: | 350/461 nm |
| Shipping Condition: | Ambient |
| Storage Conditions: | -20ºC, protect from light |
| Molecular Formula: | C27H37Cl3N6O4 |
| Molecular Weight: | 615.99 |
| CAS Number: | 23491-52-3 |
| Protocol (PDF): | C005 | C006 |
| MSDS (PDF): | MSDS-C005 | MSDS-C006 |
| COA (PDF): | C005 | C006 |
Reference:
Glycobiology (2007) 17:767-773
Product usage: Oncology, Cell Signaling, Transcription Factors, Cell Cycle, Cell Proliferation, HCS, ArrayScan, Nuclear stain
PLoS One (2012) 7:e40684-e40684
Product usage: Oncology, Cell Signaling, Transcription factors, Cellomics,Cell Proliferation, Cell cycle, Nuclear stain, C2C12 cells, Cell Signaling, Transcription factors
J Biomol Screen (2004) 9:557-568
Frequently Asked Questions (FAQs)
I have a mix of live bacteria and platelet cells, and I need to be able to separate out the bacteria. Do you have a suggestion?
Platelet cells don't have a nucleus and thus lack nuclear DNA. Therefore, a cell-permeant, DNA-selective dye would label bacteria with greater intensity relative to minor staining of the platelet mitochondrial DNA. We recommend using a Hoechst nucleic acid stain.
I need a total cell stain to label cytoplasm and nuclei in live cells. What do you recommend?
You can use a combination of two dyes with overlapping blue emission. For cytoplasm, you can label the cell with CellView Blue CMAC. This can be combined with Hoechst 33342 for nuclei. Both dyes are imaged using a standard DAPI filter set.
