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CellCycle™ Green Stain

Introduction

Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of physical, chemical, or biological means, to monitor apoptosis, and to study tumor behavior and suppressor gene mechanisms. In a given population, cells are distributed among three major phases of cell cycle: G0/G1 phase (one st of paired chromosomes per cell), S phase (DNA synthesis with variable amount of DNA), and G2/M phase (two sets of paired chromosomes per cell, prior to cell division). DNA content can be measured using fluorescent, DNA-selective stains that exhibit emission signals proportional to DNA mass. Flow cytometric analysis of these stained populations is then used to produce a frequency histogram that reveals the various cell cycle phases. This analysis is typically performed on permeabilized or fixed cells using a cell-impermeant nucleic acid stain, but is also possible using live cells and a cell-permeant nucleic acid stain.

The CellCycle™ Green stain is DNA-selective, cell membrane-permeant, and nonfluorescent stain for DNA content analysis in living cells. The CellCycle™ Green stain is fluorescent upon binding to double-stranded DNA. CellCycle™ Green stain is excited using 488 nm laser with emission ~530 nm.

The staining protocol is simple and includes incubating suspended cells in the presence of CellCycle™ stain and directly measuring the fluorescence without the need for any additional treatment or centrifugation steps. This live cell stain allows the simultaneous co-staining of the cell population for other parameters, and allows for the possibility of cell sorting based on DNA content.

 

Specifications:  
Excitation/Emission: 500/530 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 100 assays

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
A054 CellCycle™ Green Stain 500/530 100 assays $60

Documents

Protocol (PDF): A054
MSDS (PDF):  A054
COA (PDF):  A054