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CellCycle™ Green Stain


Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of physical, chemical, or biological means, to monitor apoptosis, and to study tumor behavior and suppressor gene mechanisms. In a given population, cells are distributed among three major phases of cell cycle: G0/G1 phase (one st of paired chromosomes per cell), S phase (DNA synthesis with variable amount of DNA), and G2/M phase (two sets of paired chromosomes per cell, prior to cell division). DNA content can be measured using fluorescent, DNA-selective stains that exhibit emission signals proportional to DNA mass. Flow cytometric analysis of these stained populations is then used to produce a frequency histogram that reveals the various cell cycle phases. This analysis is typically performed on permeabilized or fixed cells using a cell-impermeant nucleic acid stain, but is also possible using live cells and a cell-permeant nucleic acid stain.

The CellCycle™ Green stain is DNA-selective, cell membrane-permeant, and nonfluorescent stain for DNA content analysis in living cells. The CellCycle™ Green stain is fluorescent upon binding to double-stranded DNA. CellCycle™ Green stain is excited using 488 nm laser with emission ~530 nm.

The staining protocol is simple and includes incubating suspended cells in the presence of CellCycle™ stain and directly measuring the fluorescence without the need for any additional treatment or centrifugation steps. This live cell stain allows the simultaneous co-staining of the cell population for other parameters, and allows for the possibility of cell sorting based on DNA content.


Excitation/Emission: 500/530 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 100 assays


To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
A054 CellCycle™ Green Stain 500/530 100 assays $66



Protocol (PDF): A054
MSDS (PDF):  A054
COA (PDF):  A054

Cited Reference:

PAQR3 Inhibits Non-small Cell Lung Cancer Growth by Regulating the NF-κB/p53/Bax Axis
Guo Q, Ke X-X, Fang S-X, Gao W-L, Song Y-X, Chen C, Lu H-L and Xu G
Front. Cell Dev. Biol. 2020, 8:581919. doi: 10.3389/fcell.2020.581919

[11]-chaetoglobosins with cytotoxic activities from Pseudeurotium bakeri
Fangfang Duan, Ying Gao, Xiaogang Peng, Xianggao Meng, Jinling Chang, Yutian Gan, Qianxi Ouyang, Hanli Ruan
Bioorganic Chemistry, 2022, 127, 106011, https://doi.org/10.1016/j.bioorg.2022.106011

Overexpressed versican promoted cell multiplication, migration and invasion in gastric cancer
Zhai, L., Chen, W., Cui, B., Yu, B., Wang, Y., & Liu, H.
Tissue and Cell, 2021, 73, 101611. doi:10.1016/j.tice.2021.101611

[11]-Chaetoglobosins with Cytotoxic Activities from Pseudeurotium Bakeri
Ruan, Hanli and Duan, Fangfang and Gao, Ying and Peng, Xiaogang and Meng, Xianggao and Chang, Jinling and Gan, Yutian and Ouyang, Qianxi
​SSRN: http://dx.doi.org/10.2139/ssrn.4102564