Home » Product » Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate]

 

Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate]

Introduction

Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent Carboxy-H2DCFDA is converted to the highly fluorescent 5-(and 6)-carboxy-2',7'-dichlorofluorescein, with additional negative charges that impede its leakage out of the cell.
 

Specifications

Label: 5-(and 6)-Carboxy-2',7'-dichlorofluorescein
Ex/Em: 495/529 nm
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C25H16Cl2O9
Molecular Weight: 531.30
CAS Number:
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Probe for ROS

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C264 Carboxy-H2DCFDA 495/529 25 mg $135
 

Documents

Datasheet (PDF): C264
MSDS (PDF): MSDS-C264

References:

Myc inhibition impairs autophagosome formation.
Toh PP, Luo S, Menzies FM, Raskó T, Wanker EE, Rubinsztein DC,
Hum Mol Genet (2013) 22:5237-5248

Visualization and quantitation of cyclooxygenase-1 and -2 activity by digital fluorescence microscopy.
Ornberg RL, Koki AT
Eicosanoids and Other Bioactive Lipids in Cancer Inflammation, and Radiation Injury, 4, Honn KV, Mar 1999; (na):na pp. 131-137

Cellular carbonyl stress enhances the expression of plasminogen activator inhibitor-1 in rat white adipocytes via reactive oxygen species-dependent pathway.
Uchida Y, Ohba K, Yoshioka T, Irie K, Muraki T, Maru Y
J Biol Chem (2004) 279:4075-4083

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