Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent Carboxy-H2DCFDA is converted to the highly fluorescent 5-(and 6)-carboxy-2',7'-dichlorofluorescein, with additional negative charges that impede its leakage out of the cell.
|Storage Conditions:||-20 ℃, protect from light|
|Shipping Condition:||Room Temperature|
Probe for ROS
Hum Mol Genet (2013) 22:5237-5248
Eicosanoids and Other Bioactive Lipids in Cancer Inflammation, and Radiation Injury, 4, Honn KV, Mar 1999; (na):na pp. 131-137Product usage:
J Biol Chem (2004) 279:4075-4083