5-Aminoallyl-UTP (AA-UTP)

5-Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) (cat. no. C402) is supplied as a 10 mM solution in water.

5-Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled UTP.
 

Specifications: Concentration: 10 mM solution in water Storage: Store at -20°C Molecular Formula: C12H17N3Na3O15P3 Molecular Weight: 605.17

Applications

Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription.