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D-Biotin

Introduction

Biotin (Known as Vitamin H or Coenzyme R) is a water-soluble B-complex vitamin (vitamin B7). D-biotin binds with high affinity to streptavidin and avidin. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.

Specifications

Molecular Formula: C10H16N2O3S
Molecular Weight: 244.31
CAS Number: 58-85-5
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Biotinylation reagent

To order

Buy Cat.No. Product name Ex/Em(nm) Size Price
B001 D-Biotin 1 g $25
B002 D-Biotin   5 g $95

Documents

Datasheet (PDF): D-Biotin
MSDS (PDF): MSDS-B001, MSDS-B002

References:

Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Hirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP
Anal Biochem (2002) 308:343-357

A SPECTROPHOTOMETRIC ASSAY FOR AVIDIN AND BIOTIN BASED ON BINDING OF DYES BY AVIDIN.
GREEN NM,
Biochem J (1965) 94:23C-24C