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Andy Fluor™ 594 Alkyne

Introduction

Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
The Andy Fluor™ 594 alkyne is reactive with azide via a copper-catalyzed click reaction that allows the subsequent visualization by fluorescence spectroscopy.
 

Features

  • Efficiency—the click reaction is complete in less than 1 hour;
  • Specificity—the reaction between the label and detection tag is selective and specific;
  • Stability—the reaction product contains an irreversible, covalent bond;
  • Biologically inert—the components of the reaction do not undergo any side reactions.


 
Figure 1. Click chemistry labeling


Specifications

Label: Andy Fluor™ 594
Ex/Em: 590/615
Detection Method: Fluorescent
Solubility: DMSO, DMF
Product Size: 1 µmol
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Click chemistry labeling

 

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C321 Andy Fluor™ 594 Alkyne 590/615 1 µmol $250
 

Documents

Datasheet (PDF): C321
MSDS (PDF): C321

Reference

Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,
Angew Chem Int Ed Engl (2012) 51:3143-3146

Winz ML, Samanta A, Benzinger D, Jäschke A,
Nucleic Acids Res (2012) 40:e78-e78

Ngo JT, Schuman EM, Tirrell DA,
Proc Natl Acad Sci U S A (2013) 110:4992-4997
Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC,
J Am Chem Soc (2008) 130:11576-11577

Robust fluorescent detection of protein fatty-acylation with chemical reporters.
Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC,
J Am Chem Soc (2009) 131:4967-4975