DHE [Dihydroethidium]

Introduction

Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (Ex/Em: 355/420nm) in cells, while the oxidized form ethidium has red fluorescence (Ex/Em: 300/610 nm) upon DNA intercalation.

Specifications

Label:	Ethidium bromide
Ex/Em:	300/610 nm
Detection Method:	Fluorescent
Solubility:	DMSO, DMF
Molecular Formula:	C₂₁H₂₁N₃
Molecular Weight:	315.42
CAS Number:	38483-26-0
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications Probe for ROS

To order

Documents

Datasheet (PDF):	C260
MSDS (PDF):	MSDS-C260

References:

G2019S leucine-rich repeat kinase 2 causes uncoupling protein-mediated mitochondrial depolarization.

Papkovskaia TD, Chau KY, Inesta-Vaquera F, Papkovsky DB, Healy DG, Nishio K, Staddon J, Duchen MR, Hardy J, Schapira AH, Cooper JM,
Hum Mol Genet (2012) 21:4201-4213

Early safety assessment using cellular systems biology yields insights into mechanisms of action.

Giuliano KA, Gough AH, Taylor DL, Vernetti LA, Johnston PA,
J Biomol Screen (2010) 15:783-797

Oxidant generation by single infected monocytes after short-term fluorescence labeling of a protozoan parasite.

Chang HK, Thalhofer C, Duerkop BA, Mehling JS, Verma S, Gollob KJ, Almeida R, Wilson ME
Infect Immun (2007) 75:1017-1024

Related products

Buy	Cat.No.	Product Name	Ex/Em(nm)	Unit	Price
	C260	DHE	300/610	25 mg	$165

Related Products