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Caspase-3/7 Substrate Z-DEVD-R110


The caspase-3/7 substrate is a bisamide derivative of the fluorophore rhodamine 110 (R110). Peptides are covalently linked to each of the amino groups of rhodamine 110 (R110), thereby suppressing both the visible absorption and fluorescence of the dye. Upon enzymatic cleavage, the nonfluorescent bisamide substrate is converted in a two-step process, first to the fluorescent monoamide and then to the even more fluorescent R110. The R110 cleavage product has spectral properties similar to those of fluorescein and a large extinction coefficient, thus providing ease of use under standard fluorescein filter set-ups and excellent signal to background ratios. The substrate can be used to continuously measure enzyme activity in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader.

Substrate Specifications

Substrate name: Z-DEVD-R110
Molecular weight: 1515.5
Peptide sequence: DEVD
Useful to measure: Caspase-3, -6, -7, -8, -10
Ex/Em of R110 product: 496/520 nm
Shipping Condition: Room temperature


Assay of caspase activity.



To order

Buy Cat.No. Product Name Ex/Em Unit Price
A042 Caspase-3/7 Substrate   Z-DEVD-R110 496/520 5 mg $400



Protocol (PDF): A042
MSDS (PDF):  msds-A042


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