TUNEL Andy Fluor™ 647 Apoptosis Detection Kit

Introduction

Internucleosomal cleavage of DNA is a hallmark of apoptosis. DNA cleavage in apoptotic cells can be detected in situ in fixed cells or tissue sections using the terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) method. TUNEL is highly selective for the detection of apoptotic cells but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment.

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Hapten-tagged dUTP (e.g. digoxigenin-dUTP or biotin-dUTP) can be detected using secondary reagents (e.g. anti-digoxigenin antibodies or streptavidin) for fluorescence or colorimetric detection. Alternatively, fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry. The TUNEL Andy Fluor™ 647 Apoptosis Detection Kit contains dUTP conjugated to biotin and Streptavidin conjugated to bright and photostable Andy Fluor™ 647 far-red fluorescent dye, for bright fluorescent TUNEL staining.

Features

Broad application: Suitable for FFPE tissue, frozen tissue and cells.
High sensitivity: Can detect a low population of apoptotic cells.
Bright apoptotic signal: Uses Andy Fluor™ dyes, resulting in a stable, non-photobleaching fluorescent signal.

Specifications

Platform:	Fluorescence Microscopy
Detection Method:	Fluorescent
Ex/Em:	650/665 nm
Product Size:	50 assays
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Dry ice

Figure 1. Detection of apoptotic cells in mouse tongue tissue using TUNEL Andy Fluor™ 647 Apoptosis Detection Kit.

To order

Documents

Protocol (PDF):	A052
MSDS (PDF):	Component A Component C Component E Component G	Component B Component D Component F
COA (PDF):	COA-A052

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Wenxu Liu, Lili Wei, Meiying Li, Jingxin Mo
Materials Today Advances, 2023, 17, 100327, https://doi.org/10.1016/j.mtadv.2022.100327

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Wu, D., Zhang, Y., Zhao, C. et al.
J Transl Med 20, 583 (2022). https://doi.org/10.1186/s12967-022-03779-9

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Buy	Cat.No.	Product Name	Ex/Em	Unit	Price
	A052	TUNEL Andy Fluor™ 647 Apoptosis Detection Kit	650/665	50 assay	$420