TO3-PEG3-Biotin RNA Probe | ABP Biosciences

Introduction

TO3-PEG3-Biotin is a unique fluorogenic RNA probe. TO3-PEG3-Biotin forms a specific tight binding with the RNA Mango I Aptamer (K_D ≈ 5 nM), and results in strong fluorescence enhancement (~35X) when bound to the Mango I apatmer. This unique feature makes TO3-PEG3-Biotin as useful probe in RNA tracking and visulization. This probe is provided as 250 µM solution in DMSO.

Features:

Permeability to cell plasma and nuclear membranes.
Low toxicity to cells.
High molar absorptivity, with extinction coefficients >65,000 cm^-1 M^-1 at visible absorption maxima.
Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when unbound.
Quantum yield increases to 0.1 when bound to Mango I Aptamer.

Specifications:
Excitation/Emission:	628/648 nm
Shipping Condition:	Ambient
Storage Conditions:	-20ºC, protect from light
Molecular Formula:	C₄₆H₆₂N₆O₈S₂
Molecular Weight:	891.16
Concentration:	250 µM in DMSO

Application:	Recommended Final Concentration of Probe
In Vivo Cellular Imaging	100 nM – 200 nM
In Vitro Fluorescence Assays	100 nM – 200 nM
In Vitro Transcription (IVT)	50 nM – 200 nM
FRET Assay	50 nM – 200 nM

To Order

Documents

Protocol (PDF):	C441
MSDS (PDF):	MSDS-C441
COA (PDF):	C441

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C441	TO3-PEG3-Biotin RNA Probe	628/648	100 µL	$320