TO1-PEG3-Biotin RNA Probe | ABP Biosciences

Introduction

TO1-PEG3-Biotin is a unique fluorogenic RNA probe. TO1-PEG3-Biotin forms a specific tight binding with the RNA Mango Aptamer (K_D ≈ 3nM), and results in strong fluorescence enhancement (~1000X) when bound to the Mango apatmer. This unique feature makes TO1-PEG3-Biotin as useful probe in RNA tracking and visulization. This probe is provided as 250 µM solution in DMSO.

Features:

Permeability to cell plasma and nuclear membranes.
Low toxicity to cells.
High molar absorptivity, with extinction coefficients >65,000 cm^-1 M^-1 at visible absorption maxima.
Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when unbound.
Quantum yield increases to 0.14 when bound to Mango Aptamer.

Specifications:
Excitation/Emission:	500/530 nm
Shipping Condition:	Ambient
Storage Conditions:	-20ºC, protect from light
Molecular Formula:	C₄₁H₅₄N₆O₈S₂
Molecular Weight:	823.04
Concentration:	250 µM in DMSO

Application:	Recommended Final Concentration of Probe
In Vivo Cellular Imaging	100 nM – 200 nM
In Vitro Fluorescence Assays	100 nM – 200 nM
In Vitro Transcription (IVT)	50 nM – 200 nM
FRET Assay	50 nM – 200 nM

To Order

Documents

Protocol (PDF):	C440
MSDS (PDF):	MSDS-C440
COA (PDF):	C440

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C440	TO1-PEG3-Biotin RNA Probe	500/530	100 µL	$320