HiTrap rProtein G FF column

Introduction

HiTrap rProtein G FF columns are prepacked with cytiva rProtein G Sepharose™ Fast Flow resin for purification of monoclonal and polyclonal antibodies from most species including rat.

The recombinant protein G ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow resin by the CNBr method. The coupling technique is optimized to give high binding capacity for IgG.

The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The column can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTA™.

Features

High binding capacity: approximately 20 mg human IgG per mL resin.
Broad-spectrum: binds to a broad range of IgG species and subclasses.
Complements Protein A resins: unlike protein A, protein G binds all human and mouse IgG subclasses, including human IgG₃ and mouse IgG₁ as well as all rat IgG subclasses.
Simple operations with a syringe, pump, or high-performance chromatography system such as ÄKTA™ systems.

Table 1. Characteristics of HiTrap columns.

Table 2. HiTrap rProtein G FF characteristics

Table 3. Relative binding strengths for protein A and protein G

To Order

Documents

Protocol (PDF):	HiTrap rProtein G FF
MSDS (PDF):	MSDS-HiTrap rProtein G FF

Related Products

Buy	Cat.No.	Product name	Size	Price
	HP005	HiTrap rProtein G FF	1 × 1 mL	$220
	HP006	HiTrap rProtein G FF	1 × 5 mL	$780
	HP007	HiTrap rProtein G FF	5 × 1 mL	$880
	HP008	HiTrap rProtein G FF	5 × 5 mL	$3200