Proteinase K Solution

Proteinase K is a subtilisin-related serine protease derived from the Parengyodontium album (Tritirachium album) that is active under a wide range of reaction conditions, including elevated temperatures and the presence of Sodium Dodecyl Sulfate (SDS).

It is a broad-spectrum endopeptidase with a very high specific activity allowing highly effective digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA.

The enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product. 

Proteinase K is free of exonucleases, endonucleases, and ribonucleases.

Proteinase K solution is prepared at 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol. This Molecular Biology Grade Proteinase K solution has been sterile filtered using 0.22 μm filter, which can maintain its activity for a long time at room temperature and low temperature. It is recommended to store at -20 ℃ for long-term storage.

Applications